Evaluation of Acute and Sub-Chronic Oral Toxicity of Entoban: A Polyherbal Drug on Experimental Mice

Objectives: The current preclinical study aimed at appraising the claim of anti diarrheal activity, acute and sub chronic oral toxicity of the polyherbal formulation Entoban to acknowledge its safety and efficacy. Methods: In order to investigate the antidiarrheal activity albino mice were treated with Entoban at doses of 2.5, 5, 10 mg/kg. For evaluation of acute toxicity the animals were treated orally with doses (1 or 5 g/kg) of the Entoban capsule aqueous extract, maintained under standard laboratory conditions. Entoban was administered at doses of 50, 100 and 200 mg/kg body weight for 28 days for determining sub chronic oral toxicity. The data collected were summarized as mean ± SEM. Student’s ttest was used to determine significant differences. Results: Entoban showed significant inhibition of diarrhoea in dose dependent manner. Entoban did not cause any mortality in albino mice at the given doses of 1 or 5 g/kg. Other signs of toxicity like hair loss, weight reduction, mucus membrane (nasal), lacrimation, drowsiness, gait and tremors were also not observed. Conclusion: Results of the present study gave evidence of good tolerance of Entoban and the absence of detrimental effects on the functional state of the vital organs of the experimental animals in acute and sub chronic oral toxicity test. Future prospects include the clinical trials of the finished product as the clinical efficacy is proven in animal studies.